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KMID : 0370220030470060438
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Yakhak Hoeji
2003 Volume.47 No. 6 p.438 ~ p.443
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Production of Anti-fetuin Immnoglobulin Y and its Isolation Using an Affinity Column
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정병욱/Jung BW
정영윤/김윤중/박지훈/구완모/김병수/김기돈/김하나/Chung YY/Kim YJ/Park JH/Koo WM/Kim BS/Kim KD/Kim HN
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Abstract
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For the investigations of the usefulness of immunoglobulin Y (IgY), we attempted to produce and isolate IgY from egg yolk of white Leghorn hens immunized with fetuin as a mode l antigen. We used three methods to optimize the recovery of IgY: water dilution, polyethylene glycol, and carrageenan. The daily yield of anti-fetuin IgY from egg yolk was also examined, and it was isolated using a fetuin-affinity column at a yield of IgY of 2.7% from total IgY. Furthermore, peroxidase-labeled antifetuin IgY was prepared, and was used to determine the minimum sensitivity against fetuin, which was found to occur to a fetuin concentration of 1 ng/ml.
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